Investigating Cellular Stress Signaling Pathways

Anti-HA (Cat. number: 3724), anti-BMK1 (Cat. number: 3372), anti-phosho-ERK1/2 (Thr202/Tyr204) (Cat. number: 4376), anti-BNIP3 (Cat. number: 13795), anti-BNIP3L (Cat. number: 12396), anti-HIF1A (Cat. number: 14179), anti-Annexin V (Cat. number: 8555) and anti-ACTIN (Cat. number: 4967) antibodies were from Cell Signaling (Beverly, MA, USA). HA-tagged MEK5D-expressing vectors and BMK1 inhibitor (XMD8-92) were described in our previous study [12 (link)]. Sequence of shRNA (MISSION^® shRNA Library, sigma, USA) used in this study was described in Supplementary Table S4. pLKO.1-scramble shRNA was used as shRNAi control.

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Song C., Xu Q., Jiang K., Zhou G., Yu X., Wang L., Zhu Y., Fang L., Yu Z., Lee J.D., Yu S.C, & Yang Q. (2015). Inhibition of BMK1 pathway suppresses cancer stem cells through BNIP3 and BNIP3L. Oncotarget, 6(32), 33279-33289.

Publication 2015

Anti-HA anti-BMK1 anti-phosho-ERK1/2 (Thr202/Tyr204) anti-BNIP3 anti-BNIP3L anti-HIF1A anti-Annexin V anti-ACTIN MISSION® shRNA Library

Actin Annexin v Antibodies Erk1 Hif1a Library Shrna Vectors Xmd8 92

Corresponding Organization :

Other organizations : Scripps Research Institute, Dalian Medical University, Army Medical University, Southwest Hospital

Top 5 similar protocols

Variable analysis

independent variables

HA-tagged MEK5D-expressing vectors
BMK1 inhibitor (XMD8-92)

dependent variables

Phospho-ERK1/2 (Thr202/Tyr204)
BNIP3
BNIP3L
HIF1A
Annexin V
ACTIN

control variables

PLKO.1-scramble shRNA

controls

Positive controls: Not specified
Negative controls: pLKO.1-scramble shRNA

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