In all studies, the human-derived, PSMA-expressing prostate cancer tumor cell line C4-2 was used (courtesy of Dr. George Thalmann, Inselspital Bern, Switzerland). Cells were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (Omega Scientific) and grown at 37°C and 5% CO2. Cells were monitored for Mycoplasma contamination using the Venor GeM Mycoplasma detection kit (Sigma Aldrich) and authenticated by short tandem repeat sequencing (Laragen). The parental cells were engineered to express firefly luciferase (C4-2-luc) to allow luciferase-mediated bioluminescence imaging to monitor tumor burden, as previously described (41 (link)).
All animal studies were approved by the UCLA Animal Research Committee (approval 2005-090). The mice were housed under pathogen-free conditions with food and water ad libitum and a 12 h–12 h light–dark cycle. Veterinary staff and investigators observed the mice daily to ensure animal welfare.
All animal studies were approved by the UCLA Animal Research Committee (approval 2005-090). The mice were housed under pathogen-free conditions with food and water ad libitum and a 12 h–12 h light–dark cycle. Veterinary staff and investigators observed the mice daily to ensure animal welfare.
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