Cloning and Characterization of NR4A1 Variants

The Flag-tagged full-length FLAG-NR4A1 expression plasmids were constructed by inserting PCR-amplified full-length NR4A1 (amino acids 1–599) into the EcoRI/BamHI site and C-terminal NR4A1 (amino acids 67–599) and N-terminal NR4A1 (amino acids 1–354) into the EcoRI/KpmI site of the p3XFLAG-CMV-10 expression vector (Sigma-Aldrich). NBRE_x3-luc was generously provided by Dr Jacques Drouin (University of Montreal, Montreal, QC, Canada). All other plasmids used in this study were previously described [21 (link),22 (link)].

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Karki K., Mohankumar K., Schoeller A., Martin G., Shrestha R, & Safe S. (2021). NR4A1 Ligands as Potent Inhibitors of Breast Cancer Cell and Tumor Growth. Cancers, 13(11), 2682.

Publication 2021

p3XFLAG-CMV-10 expression vector

Amino acids Ecori Nr4a1 Plasmids Vector

Corresponding Organization : Texas A&M University

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Variable analysis

independent variables

Full-length FLAG-NR4A1 expression plasmids
C-terminal NR4A1 (amino acids 67-599) expression plasmids
N-terminal NR4A1 (amino acids 1-354) expression plasmids

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

NBRE_x3-luc reporter plasmid (positive control)

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