HT-22 and SH-SY5Y cells were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin–streptomycin (HyClone, Logan, UT, USA). All the cells were incubated at 37 °C in a humidified incubator containing 5% CO2. For cell transfection, the cells were transfected with shNC and shJWA plasmids for 48 h using lipo 8000 reagent (Beyotime, Shanghai, China). The shJWA plasmid’s construction was conducted with reference to the previous research conducted in our laboratory [16 (link)].
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