Cisplatin-Induced Hearing Loss in Mice

ABR tests were performed in 2–3 months old mice 1 week before the first cisplatin injection. Following the ABR tests, mice received 2–3 ml saline (Teknova, Fishersci) 1 day before the first cisplatin injection. cisplatin (100 mg/ml; APP pharmaceuticals) was administered i.p. at 3 mg/kg each day for 4 days using a modified cisplatin protocol described by Lisa Cunningham’s group^{19 (link),23 (link)}. Following this 4-day cisplatin injection period, mice recovered for 10 days. Mice received 2–3 ml saline daily during the 4-day cisplatin injection period and daily during the 10-day recovery period. Mice were also provided with a high calorie liquid diet (STAT, PRN Pharmacal) and bacon softies (BioServ) daily. This 14-day protocol was repeated once more for a total of two cycles of cisplatin administration. Mice had an additional 7 days of recovery following the final cisplatin injection before the postcisplatin treatment ABR tests. To promote exercise and facilitate the study of healthy animals, mice were housed in cages with mouse Igloos (hut) with attached wheel (BioServ) throughout the cisplatin administration protocol.