CMPs were synthesized on glycine‐preloaded Wang or polyethylene glycol‐based resins using Fmoc‐protected monomers from Chem‐Impex, as described previously.[19 (link)
] Condensation of Fmoc‐ProHypGly‐OH,[11b (link)
] Fmoc‐GlyProHyp‐OH (Bachem), or Fmoc‐GlyPro‐OH (Chem‐Impex) segments was employed wherever applicable. Isolated crude peptides were purified, as described previously,[16 (link)
] to >95% purity according to analytical HPLC and MALDI–TOF mass spectrometry (MS). MS analyses were carried out on an Applied Biosystems Voyager DE‐Pro mass spectrometer at the University of Wisconsin–Madison Biophysics Instrumentation Facility (UW–BIF;www.biochem.wisc.edu/bif ). m/z calcd for 11/3sb variants [M + H]+, 3057.2; found, 3057.1 for OKD; found, 3057.4 for KDO; found, 3057.0 for DOK; found, 3057.3 for DOKctrl. Analytical HPLC results for purified CMPs are shown in Figure S1 (Supporting Information). m/z calcd for 8/2sb‐OKD [M + H]+, 2222.3; found, 2222.4. Analytical HPLC chromatograms of purified peptides are presented in Figures S1 and S2A (Supporting Information).
] Condensation of Fmoc‐ProHypGly‐OH,[11b (link)
] Fmoc‐GlyProHyp‐OH (Bachem), or Fmoc‐GlyPro‐OH (Chem‐Impex) segments was employed wherever applicable. Isolated crude peptides were purified, as described previously,[16 (link)
] to >95% purity according to analytical HPLC and MALDI–TOF mass spectrometry (MS). MS analyses were carried out on an Applied Biosystems Voyager DE‐Pro mass spectrometer at the University of Wisconsin–Madison Biophysics Instrumentation Facility (UW–BIF;
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