Lipid Mediators and Signaling Pathways

BP was obtained from Sigma-Aldrich (St. Louis, MO, USA). MC was obtained from Toronto Research Chemicals (Cat No. M294460, Toronto, ON, USA). Fish oil (from menhaden) was also purchased from Sigma-Aldrich (St. Louis, MO, USA). Omega-3 fatty acids EPA and DHA were purchased from Cayman Chemical (Ann Arbor, MI, USA). sEH inhibitors Trans-4-[4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid (t-TUCB) and 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (TPPU) were kindly provided by Dr. Bruce Hammock (UC, Davis). CYP1B1 antibody was generously provided by Dr. Colin Jefcoate (University of Wisconsin-Madison). GRP78 polyclonal antibodies were purchased from Abcam (Cat. No. ab21685, Waltham, MA, USA). Materials for DNA extraction [80 (link),81 (link),82 (link),83 (link)], RNA extraction [84 (link)], and ³²P-postlabeling analysis [80 (link),85 (link),86 (link)] have been reported previously.