Viral Progeny Production Assay

The production of viral progeny was measured in RK‐13 cells using the Reed–Muench method as described previously (1 (link)). Briefly, the cells were grown to approximately 80%–90% confluence in 96-well cell plates (Corning, USA). The viral supernatants were serially diluted 10-fold, and 100 µL of each dilution was added to each well. After 1 h of virus adsorption, the cells were washed with PBS and incubated in 3% FBS DMEM. Five days post-infection, the TCID₅₀ was calculated and analyzed using GraphPad 8.0.

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Wang T., Hu L., Li R., Ren H., Li S., Sun Q., Ding X., Li Y., Wang C, & Li L. (2024). Hyperoside inhibits EHV-8 infection via alleviating oxidative stress and IFN production through activating JNK/Keap1/Nrf2/HO-1 signaling pathways. Journal of Virology, 98(4), e00159-24.

Publication 2024

96-well cell plates

Corresponding Organization :

Other organizations : Liaocheng University, Qingdao Agricultural University

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Variable analysis

independent variables

Viral supernatant dilution

dependent variables

TCID50 (Tissue Culture Infectious Dose 50)

control variables

RK-13 cell line
Cell confluence (80%-90%)
Cell culture medium (3% FBS DMEM)
Incubation time (5 days post-infection)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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