Plasmid Expression Vectors for Brd4 and JMJD6

pET-28a-JMJD6, pBobi-3
× FLAG-3 × HA-JMJD6, pcDNA4C-his-Brd4 (full-length), pcDNA4C-his-Brd4
(1–470), pcDNA4C-his-Brd4 (471–730), pcDNA4C-his-Brd4
(731–1046), and pcDNA4C-his-Brd4 (1047–1362) expression
vectors were reported previously.^{28 (link),29 (link)} BRD4 ET (601–683)
was PCR-amplified from full-length BRD4 by using PrimeSTAR HS DNA
Polymerase (Takara) and then cloned into pGEX-6P-1 (Promega) expression
vectors. JMJD6 PiET deletion (Δ95–103) was PCR-amplified
from full-length JMJD6 by using PrimeSTAR HS DNA Polymerase and then
cloned into pET-28a (Promega) expression vectors.

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Huang Q.X., Fan D.M., Zheng Z.Z., Ran T., Bai A., Xiao R.Q., Hu G.S, & Liu W. (2024). Peptide Inhibitor Targeting the Extraterminal Domain in BRD4 Potently Suppresses Breast Cancer Both In Vitro and In Vivo. Journal of Medicinal Chemistry, 67(8), 6658-6672.

Publication 2024

PrimeSTAR HS DNAPolymerase pGEX-6P-1 pET-28a

Corresponding Organization : Xiamen University

Other organizations : Guangzhou Regenerative Medicine and Health Guangdong Laboratory

Top 5 similar protocols

Variable analysis

independent variables

PET-28a-JMJD6
PBobi-3 × FLAG-3 × HA-JMJD6
PcDNA4C-his-Brd4 (full-length)
PcDNA4C-his-Brd4 (1–470)
PcDNA4C-his-Brd4 (471–730)
PcDNA4C-his-Brd4 (731–1046)
PcDNA4C-his-Brd4 (1047–1362)
BRD4 ET (601–683)
JMJD6 PiET deletion (Δ95–103)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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