MCF-10A-ER-Src Cell Transformation Protocol

MCF-10A-ER-Src cells (Iliopoulos et al., 2009 (link); Iliopoulos et al., 2010 (link); Ji et al., 2018 (link); Ji et al., 2019 (link)) were grown in DMEM/F12 without phenol red (Thermo Fisher Scientific, 11039–047) + 5% charcoal stripped FBS (Sigma, F6765) + 1% pen/strep (Thermo Fisher Scientific, 15140122) + 20 ng/ml EGF (Peprotech, AF-100–15) + 0.5 μg/ml Hydrocortisone (Sigma, H-0888) + 0.1 μg/ml cholera toxin (Sigma, C-8052) + 10 μg/ml insulin (Sigma, 10516). 1–0.4 μM Tamoxifen (Sigma, H7904) + 2–4 μM AZD0530 (Selleck Chemicals, S1006) were used to induce the transformation. MDA-MB-231 cells were grown in DMEM (Thermo Fisher Scientific, 11995–073) + 10% FBS (Sigma, TMS-013-B) + 1% pen/strep.