Quantifying Starch Composition: Amylose and Amylopectin

Amylose content was measured using a continuous flow analytical system (Skalar San⁺⁺ System, Netherlands). Standards were prepared using potato amylose (Solarbio, China) with a concentration gradient of 2%, 4%, 8%, 12%, 16%, 20%, and 32%. The absorbance of amylose content was determined at 600 nm. Total starch content was measured spectrophotometrically based on the determination of the absorbance of glucose concentration at 540 nm (Li and Peng, 2018 (link)). According to the transformational equation between starch and glucose, total starch content was calculated by the following formula: Total starch content (mg·g^-1) = glucose concentration × 10 × 9.11 ml × 0.9/(50 × 1000). Amylopectin content = total starch content - amylose content (Prathap et al., 2019 (link)).