The qPCR experiments were performed as described previously [19 (link), 20 (link)]. The total RNA (Ribonucleic Acid) of cells were extracted using the Trizol buffer (Beyotime) and were reverse-transcribed by Multiscribe™ Reverse Transcriptase (Applied Biosystems, Thermo Scientific Corporation) according to the manufacturer’s instructions. The relative expression was calculated by the comparative Ct method. The primers used for qPCR analysis were shown in Table 1 .
The primers used in this work
| Targets | Primer sequence (5′–3′) |
|---|---|
| Forward: 5′-GGGACGCAGACATCGTCATC-3′ | |
| Reverse: 5′-TCGTCATCGTCGAAATGGGC-3′ | |
| Forward: 5′-TGAAGCGACTGATGTCCCTG-3′ | |
| Reverse: 5′-CAAAGATGGTCACGGTCTGC-3′ | |
| Forward: 5′-CCTGGTTCATCCAGTCGCTT-3′ | |
| Reverse: 5′-TCTGTTGCCACCTTTCGGTT-3′ | |
| Forward: 5′-GTGAAGTCAACATGCCTGCC-3′ | |
| Reverse: 5′-ACAGCCTGCAGCTTTGTTTC-3′ |
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