Swarming motility assays were performed on minimal medium (M8) plates with an agar concentration of 0.6 % (w/v) [45 (link)]. To assess the swarming motility 2 µl of a P. aeruginosa culture containing approx. 1×109 c.f.u. ml−1 was put on the centre of the agar. The plates were incubated at 37 °C during 24 h after which the diameter was measured.
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