Murine Myeloid Cell Isolation Protocol

Estradiol benzoate (HY-B1192) was purchased from MCE. Gox (G7141), collagenase type IV (C5138) and DNase I (11284932001) were purchased from Sigma-Aldrich. Fluorochrome-labeled or unlabeled monoclonal antibodies against mouse Ly6G (108454, 127607), IgG2b isotype control (400675), CD16/32 (101319), CD45 (103113), CD11b (101227), F4/80 (123115), FcγR III (158013) were purchased from BioLegend. Recombinant anti-myeloperoxidase antibody (ab208670) was purchased from Abcam. DMEM/F-12 GlutaMAX (SH30023.01) was purchased from Hyclone. 2-NBDG (N13195) was purchased from Invitrogen. Cell Counting Kit-8 (E-CK-A362) was purchased from Elabscience. BSA-FITC, BSA-ICG were purchased from Xi’an ruixi Biological Technology Co., Ltd.

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Zhu S., Zhang J., Xue N., Zhu X., Li F., Dai Q., Qing X., Chen D., Liu X., Wei Z, & Cao Y. (2023). Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. Journal of Nanobiotechnology, 21, 81.

Publication 2023

DNase I

2 nbdg Anti antibody Biological Cd11b Collagenase Dnase i Estradiol benzoate Fitc Fluorochrome Igg2b Isotype Monoclonal antibodies Mouse Myeloperoxidase

Corresponding Organization : First Affiliated Hospital of Anhui Medical University

Other organizations : Hefei National Center for Physical Sciences at Nanoscale, University of Science and Technology of China

Top 5 similar protocols

Variable analysis

independent variables

Estradiol benzoate (HY-B1192)

dependent variables

Cell Counting Kit-8 (E-CK-A362)
2-NBDG (N13195)

control variables

Gox (G7141)
Collagenase type IV (C5138)
DNase I (11284932001)
Fluorochrome-labeled or unlabeled monoclonal antibodies against mouse Ly6G (108454, 127607), IgG2b isotype control (400675), CD16/32 (101319), CD45 (103113), CD11b (101227), F4/80 (123115), FcγR III (158013)
Recombinant anti-myeloperoxidase antibody (ab208670)
DMEM/F-12 GlutaMAX (SH30023.01)
BSA-FITC
BSA-ICG

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