Splenic Lipid Peroxidation Assay

At 9.5 weeks old, the mice were anesthetized, and their blood samples were collected by cardiac puncture and placed in EDTA containing tubes. The animals were sacrificed under anesthesia with 1% ketamine. and their spleens were excised and weighed. A portion of the spleen was washed with the washing buffer and homogenized, and the supernatant obtained was used for lipid peroxidation assay. A portion of the splenic tissue was fixed in 10% formalin solution and stored for histological analysis.

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Al-Griw M.A., Balog H.N., Shaibi T., Elmoaket M.F., AbuGamja I.S., AlBadawi A.B., Shamlan G., Alfarga A., Eskandrani A.A., Alnajeebi A.M., Babteen N.A., Alansari W.S, & Alghazeer R. (2023). Therapeutic potential of vitamin D against bisphenol A-induced spleen injury in Swiss albino mice. PLOS ONE, 18(3), e0280719.

Publication 2023

Anesthesia Animals Assay Blood Buffer Cardiac Edta Formalin Ketamine Lipid peroxidation Mice Puncture Splenic

Corresponding Organization : Taibah University

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Variable analysis

independent variables

Age of mice (9.5 weeks old)

dependent variables

Spleen weight
Lipid peroxidation in the spleen

control variables

Anesthesia (1% ketamine)
Cardiac puncture for blood collection
Formalin fixation of splenic tissue for histological analysis

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