Plasma and Erythrocyte Oxidative Markers

Blood samples were collected after decapitation in a vacutainer tube containing EDTA as an anticoagulant for the assay of pro-oxidative markers in the plasma and antioxidant markers in the lysate. The samples were centrifuged at 3000 rpm for 10 min at 4 °C using a Centurion centrifuge (K280R, UK). The plasma and erythrocyte lysate were then stored at -20 °C until analysis. All measurements were performed spectrophotometrically (Shimadzu UV-1800, Japan).

Free full text: Click here

Radoman K., Zivkovic V., Zdravkovic N., Chichkova N.V., Bolevich S, & Jakovljevic V. (2023). Effects of dandelion root on rat heart function and oxidative status. BMC Complementary Medicine and Therapies, 23, 78.

Publication 2023

UV-1800

Anticoagulant Antioxidant Assay Blood Decapitation Edta Erythrocyte Plasma

Corresponding Organization :

Other organizations : Faculty for State and European Studies, University of Kragujevac, Sechenov University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Pro-oxidative markers in the plasma
Antioxidant markers in the erythrocyte lysate

control variables

Vacutainer tube containing EDTA as an anticoagulant
Centrifugation at 3000 rpm for 10 min at 4 °C using a Centurion centrifuge (K280R, UK)
Storage of plasma and erythrocyte lysate at -20 °C until analysis
Spectrophotometric measurements using Shimadzu UV-1800, Japan

controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!