The macrocyclic chelator, 1,4,7-triaza cyclononane-N, N', N”-triacetic acid (NOTA) conjugated BBN-RGD was synthesized according to a method described in our previous publication 17 (link). 68Ga-BBN-RGD was prepared following the procedure reported previously 24 (link). The radiochemical purity was greater than 95%.
No specific subject preparation, such as fasting, was requested on the day of 68Ga-BBN-RGD PET/CT. Each patient was intravenously injected with 68Ga-BBN-RGD in a dosage of approximately 1.85 MBq (0.05 mCi) per kilogram of body weight, ranging from 75.9 to 148.0 (114.7 ± 17.1) MBq. PET/CT was performed at 25~35 min after tracer administration by using Biograph 64 Truepoint TrueV system (Siemens Medical Solutions, Knoxville, TN, USA). After a low-dose CT scan (120 kV, 35 mA, 3 mm layer, 512 × 512 matrix, 70 cm FOV), whole body PET acquisition was performed with 2 min per bed position (five to six bed positions depending on the height of the patient). The emission data were corrected for randoms, dead time, scattering, and attenuation. The conventional reconstruction algorithm was used and the images were zoomed with a factor of 1.2. The images were transferred to a MMWP workstation (Siemens) for analysis.
No specific subject preparation, such as fasting, was requested on the day of 68Ga-BBN-RGD PET/CT. Each patient was intravenously injected with 68Ga-BBN-RGD in a dosage of approximately 1.85 MBq (0.05 mCi) per kilogram of body weight, ranging from 75.9 to 148.0 (114.7 ± 17.1) MBq. PET/CT was performed at 25~35 min after tracer administration by using Biograph 64 Truepoint TrueV system (Siemens Medical Solutions, Knoxville, TN, USA). After a low-dose CT scan (120 kV, 35 mA, 3 mm layer, 512 × 512 matrix, 70 cm FOV), whole body PET acquisition was performed with 2 min per bed position (five to six bed positions depending on the height of the patient). The emission data were corrected for randoms, dead time, scattering, and attenuation. The conventional reconstruction algorithm was used and the images were zoomed with a factor of 1.2. The images were transferred to a MMWP workstation (Siemens) for analysis.
