Several Biomphalaria glabrata snails were kindly provided by Dr. Yousheng Liang from Jiangsu Provincial Institute of Schistosomiasis Control and Prevention. The Puerto Rico (PR) strain of B. glabrata has been kept and bred in Dr. Liang’s laboratory for 10 years. Snails were continued to produce for two generations in our laboratory before snails collection in this study.
Snails ranging from 10.0 mm to 15.0 mm in shell diameter were randomly selected, placed in a clean container with 1 L aerated dechlorinated tapwater per 15 snails, and reared at 26–28 °C with a 12 h light and 12 h dark photocycle and fed with fresh green-leaf lettuce. The container was cleaned regularly to remove food residue. Small clean cling films were set on the water surface to let snails deposit eggs. The egg masses were collected daily and transferred to a clean container with dechlorinated tap water. The newly hatched snails were kept in 200 ml water in a shallow 500 ml container per 100 snails and fed with dried lettuce and fish food. The juvenile and adult snails were continually transferred to a new container with 1–1.5 L of dechlorinated tap water, fed, and maintained under the same conditions as the above procedure.
Snails ranging from 10.0 mm to 15.0 mm in shell diameter were randomly selected, placed in a clean container with 1 L aerated dechlorinated tapwater per 15 snails, and reared at 26–28 °C with a 12 h light and 12 h dark photocycle and fed with fresh green-leaf lettuce. The container was cleaned regularly to remove food residue. Small clean cling films were set on the water surface to let snails deposit eggs. The egg masses were collected daily and transferred to a clean container with dechlorinated tap water. The newly hatched snails were kept in 200 ml water in a shallow 500 ml container per 100 snails and fed with dried lettuce and fish food. The juvenile and adult snails were continually transferred to a new container with 1–1.5 L of dechlorinated tap water, fed, and maintained under the same conditions as the above procedure.
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