All plant materials of Dipterocarpus turbinatus Gaertn. f. (HCNGB_00001637) and Hopea hainanensis Merr. et Chun. (HCNGB_00001636) used in this study were collected from Ruili Botanical Garden (Yunnan, China). High‐quality DNA was extracted from fresh leaves by using QIAGEN® Genomic kits, and the DNA quantification was checked by Nanodrop and Qubit. PromethION Nanopore sequencer with the long‐read DNA sequencing type was used for genome sequencing. The SQK_LSK109 Ligation Sequencing Kit was used to prepare the sequencing libraries. Finally, a total of 27 and 26 Gb pass reads were generated for D. turbinatus and H. hainanensis, respectively.
For short read Illumina sequencing, the genomic DNA was isolated from fresh leaves using a modified CTAB protocol (Sahu and Thangaraj, 2012 (link)). The extracted DNA was used to create four paired‐end libraries (170, 350, 500, and 800 bp) and four mate‐pair libraries (2, 6, 10 and 20 Kb) using the Illumina standard methods (San Diego, CA). Following that, the sequencing was performed by employing a whole‐genome shotgun sequencing approach on an Illumina HiSeq 2000 platform (San Diego).
For short read Illumina sequencing, the genomic DNA was isolated from fresh leaves using a modified CTAB protocol (Sahu and Thangaraj, 2012 (link)). The extracted DNA was used to create four paired‐end libraries (170, 350, 500, and 800 bp) and four mate‐pair libraries (2, 6, 10 and 20 Kb) using the Illumina standard methods (San Diego, CA). Following that, the sequencing was performed by employing a whole‐genome shotgun sequencing approach on an Illumina HiSeq 2000 platform (San Diego).
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