The igVR-1a chimera gene, GND, and JFH1 vectors were digested with the XbaI restriction enzyme (Roche, USA). For in vitro RNA transcription, mung bean nuclease catalyst was used in the degradation of single-stranded DNA and RNA (Promega, USA). In vitro RNA transcription was performed with a MEGAscript T7 kit (Ambion, Germany) according to the manufacturer’s recommendations. After DNase I treatment, RNA was used for transfection (12 (link)).
Protocol detail
Chimeric Gene Transcription Protocol
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Chimera
Dnase i
Gene
Mung bean nuclease
Promega
Restriction enzyme
Single stranded dna
Transcription
Transfection
Vectors
Corresponding Organization : Hormozgan University of Medical Sciences
Other organizations : University of Aberdeen, Academic Center for Education, Culture and Research, Pasteur Institute of Iran
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