RAW 264.7 cells were treated with the indicated concentrations of silibinin in the presence of LPS (200 ng/ml) for 18 h. Culture supernatants were collected, and the accumulation of NO2- in culture supernatants was measured as an indicator of NO production in the medium as previously described (Green et al., 1982 (link); Huong et al., 2012) (link).
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Silibinin Modulates NO Production in LPS-Stimulated RAW 264.7 Cells
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Raw 264 7 cells
Silibinin
Corresponding Organization :
Other organizations : Chosun University, Jeju National University
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Variable analysis
independent variables
- Silibinin concentrations
- Accumulation of NO2- in culture supernatants
- Presence of LPS (200 ng/ml)
- Positive control: Cells treated with LPS (200 ng/ml) without silibinin
- Negative control: Cells not treated with LPS or silibinin
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