To measure antibody-secreting cells in the bone marrow, cells isolated from bone marrow aspirates by density gradient centrifugation using Lympholyte Mammal (Cedarlane Laboratories) were incubated for 6 hours with Multiscreen ELISpot plates (MilliporeSigma) coated with MeV-infected Vero cell lysate, H-expressing L cell lysate, or baculovirus-expressed N. Bound immunoglobulin was detected with horseradish peroxidase (HRP)–conjugated goat anti-monkey IgG (Nordic), developed with stable diaminobenzidine (DAB) solution and read on an ImmunoSpot plate reader (Cellular Technology).
Measuring Measles Virus Antibody Response
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Variable analysis
- Serially diluted plasma
- Plaque formation on Vero cells
- MeV-specific IgG and IgA levels in plasma
- H-specific IgG levels in plasma
- Avidity of MeV-specific IgG in plasma
- Antibody-secreting cells in bone marrow
- Chicago-1 strain of MeV
- Vero cells
- MeV-infected Vero cell lysate
- MeV H-expressing L cells
- Baculovirus-expressed N
- Lympholyte Mammal for bone marrow cell isolation
- Multiscreen ELISpot plates
- Not specified
- Not specified
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