To compare tissue transcriptomes of A. cerana and A. mellifera, RNAseq reads from antenna tissue of each species were mapped against annotated Gr, Or, and Ir gene sequences using RSEM [113 (link)]. TMM (Trimmed Mean of M-values)-normalized FPKM (fragments per kilobase per million) expression values were calculated by running perl scripts included in Trinity assembler (version r2013-02-25) and were used to draw a heat map [98 (link)].
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