Subjects wore an Actigraph (wGT3X-BT or wActisleep-BT, Pensacola, FL) on the non-dominant wrist for 7 days to quantify their physical activities. Subjects were instructed to complete daily logs regarding sleep time, physical activity, and any removal time. Data were downloaded and analyzed via Actilife software (Actilife 6.13.1, Pensacola, FL). Troiano algorithm and the daily logs were used to identify and remove the non-wear and sleep time from daytime physical activity calculation. The data of four valid days (wear time of at least 10 waking hours) were used for all subjects, which have been shown to be a representative for the data of 1 week [26 (link)]. Activities were divided into either sedentary/light activities or moderate to vigorous physical activities (MVPA) based on Freedson criteria [11 (link), 12 (link), 36 (link)].
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