Chemical Compound Preparation Protocol

Solutions of potassium cyanide (Sigma Aldrich 60178-25G), sodium chloride (BioShop SOD002.205), cycloheximide (Sigma Aldrich C7698-1G), abamectin (Sigma Aldrich 31732) and 2-deoxy-D-glucose (Sigma Aldrich D8375) were prepared fresh to twice the working concentration in M9 buffer, 1.6% DMSO. Stock solutions of aldicarb (Sigma Aldrich, 33386) were prepared to 1 M in DMSO. Solutions of levamisole hydrochloride (Acros Organics, 16595-80-5), oxotremorine M (Sigma Aldrich, O100) and mecamylamine hydrochloride (Sigma Aldrich, M9020) were prepared to 100 mM in water. These solutions were divided into single use aliquots and frozen. Stocks of arecoline hydrobromide (Acros Organics, 250130050) and atropine sulfate monohydrate (Sigma Aldrich, A0257) were prepared fresh on the day of use in M9 buffer. (-)-Nicotine (Sigma Aldrich, N3876) was stored frozen, undiluted in single use aliquots. Compounds described in Burns et al. (2015) (link) were purchased as a custom library of 10 mM stock in DMSO (ChemBridge Corporation) and stored frozen.