Antibiotic Modulation of Intestinal Inflammation in Apc-Mutant Mice

Apc^Min/+ mice were pretreated with an antibiotics (ABX) mixture containing 500 mg/l vancomycin (Cat# V820413), 1 g/l ampicillin (Cat# A6265), 1 g/l metronidazole (Cat# M813526) and 1 g/l neomycin (Cat# N814740) (VAMN, Macklin Inc., Shanghai, China) for 3 weeks with addition of 75 g/l sucrosein drinking water, and then were co-administrated with DSS for 1 week, and were continuously treated with ABX for further 4 weeks. In some experiment, ABX cocktail containing 0.4 g/l kanamycin (Cat# K812216), 0.035 g/l gentamycin (Cat# G6064), 0.0570 g/l colistin (Cat# C805491), 0.215 g/l metronidazole, 0.045 g/l vancomycin HCl and 0.025 g/l erythromycin (Cat# E808820)^{68 (link)} (6 mixture, Macklin) were used to treat mice.