Protocol detail

Plasmid Constructs for CLC-1, FKBP8, and More

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Flag-CLC-1 was generated by subcloning human CLC-1 into the pFlag-CMV2 vector (Sigma-Aldrich, St. Louis, MO, USA) [18 (link)]. Myc-FKBP8 was created by swapping mouse FKBP8 into the pcDNA3.1-Myc vector (Sigma-Aldrich) [19 (link)]. Additional cDNA constructs employed in this study are pcDNA3.1-Myc mouse Aha1, pcDNA3-HA rat HOP, pcDNA5-V5 human Hsc70 (Addgene 19514, Watertown, MA, USA), pcDNA3-HA human Hsp90β (Addgene 22847), and pEGFP-rat TGN38 (kindly provided by Dr. Fan-Jen Lee, National Taiwan University, Taipei, Taiwan).

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Peng Y.J., Lee Y.C., Fu S.J., Chien Y.C., Liao Y.F., Chen T.Y., Jeng C.J, & Tang C.Y. (2018). FKBP8 Enhances Protein Stability of the CLC-1 Chloride Channel at the Plasma Membrane. International Journal of Molecular Sciences, 19(12), 3783.

Publication 2018

pFlag-CMV2 vector pcDNA3-HA rat HOP pcDNA5-V5 human Hsc70 pcDNA3-HA human Hsp90β

Cdna Fkbp8 Human Mouse Vector

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Variable analysis

independent variables

Flag-CLC-1
Myc-FKBP8
PcDNA3.1-Myc mouse Aha1
PcDNA3-HA rat HOP
PcDNA5-V5 human Hsc70
PcDNA3-HA human Hsp90β
PEGFP-rat TGN38

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned.

Annotations

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