Targeted Metabolomics by LC-MS and GC-MS

Targeted metabolomics was performed as described in detail before with liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry.²² (link)^,³⁷ (link) A total of 133 metabolites that cover major metabolic pathways were quantified (see detailed pathways and parameters in Supplementary Table S1). A Shimadzu LC Nexera X2 UHPLC coupled with a QTRAP 5500 liquid chromatography-mass spectrometry (AB Sciex, Hong Kong), and an Agilent 7890B/5977B gas chromatography-mass spectrometer (Agilent Technologies, Santa Clara, CA. USA) were used for metabolite analysis. The data were analyzed by MultiQuant 3.0.2 (AB Sciex) and Agilent MassHunter Quantitative Analysis Software.³⁹ (link)

Free full text: Click here

Saravanan M., Xu R., Roby O., Wang Y., Zhu S., Lu A, & Du J. (2023). Tissue-Specific Sex Difference in Mouse Eye and Brain Metabolome Under Fed and Fasted States. Investigative Ophthalmology & Visual Science, 64(3), 18.

Publication 2023

LC Nexera X2 UHPLC QTRAP 5500 MultiQuant 3.0.2 MassHunter Quantitative Analysis Software

Gas chromatography Gas chromatography mass spectrometry Liquid chromatography Mass spectrometry

Corresponding Organization : West Virginia University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

133 metabolites that cover major metabolic pathways

control variables

None explicitly mentioned

controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!