Time spent on sedentary behaviors (≤ 99 cpm) was measured by a triaxial accelerometer (ActiGraph GT3X+) worn on the waist [22 (link)]. A break in a sedentary time was defined as at least 1 min when the accelerometer registered ≥100 cpm following a sedentary period, according to previous definitions [23 (link), 24 (link)]. We followed the data collection and processing criteria procedure suggested by a systematic review of standard protocols for the use of accelerometers [25 (link)]. Non-wear time was identified while the periods of more than 60 consecutive minutes of zero counts. The participants were also asked to assess their sleep time and duration using a sleep log. Time intervals during the waking hours were identified, in which sedentary breaks occurred in the morning (06:00–12:00), afternoon (12:00–18:00) and evening (18:00–24:00) based on previous studies [26 (link), 27 (link)]. ActiLife software version 6.0 was used to extract the accelerometer data; all data were processed using 60-s time-spans with a default sampling frequency of 30 Hz.
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