Protein Expression Analysis by Western Blot

Whole cell lysates and proteins from tissue were harvested and subjected to western blotting using the following primary antibodies: SNAIL (1:500, #3895, Cell Signaling Technology), E-cadherin (1:2000, #610181, BD Biosciences), HSP90 (1:250, #sc-13119, Santa Cruz Biotechnology), TRP53 (1:1000, #NCL-p53-CM5p, Novocastra/Leica Microsystems), p21^CIP1 (1:200, #sc-397, Santa Cruz Biotechnology), β-Actin (1:4000, #A5316, Sigma-Aldrich) and α-Tubulin (1:5000, #T9026, Sigma-Aldrich). The western blot images were collected using the Odyssey infrared imaging system with the Odyssey Software V1.2 (Li-Cor Biosciences).