Adoptive Transfer of CFSE-Labeled OTI T Cells

CD8⁺ T cells from spleens and LNs of OTI × B6.SJL-Ptprca mice were positively selected using anti-CD8⁺ MACS Beads (Miltenyi Biotec) and labeled with CFSE as described (32 (link)). Purity was >87% as assessed by positive staining with anti-CD8α, -Vα2, and -Vβ5 antibodies. About 2 × 10⁶ cells were injected i.v. into C57BL/6J or CD11c-DTR BM chimeric hosts bearing established B16.OVA tumors. OTI proliferation was assessed in tumor-draining LNs 6 days later.

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Kuhn S., Yang J, & Ronchese F. (2015). Monocyte-Derived Dendritic Cells Are Essential for CD8+ T Cell Activation and Antitumor Responses After Local Immunotherapy. Frontiers in Immunology, 6, 584.

Publication 2015

anti-CD8+ MACS Beads

Antibodies Cd8 t cells Cells Cfse Chimeric Mice Tumor

Corresponding Organization :

Other organizations : Malaghan Institute of Medical Research

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Variable analysis

independent variables

Host mouse genotype (C57BL/6J or CD11c-DTR BM chimeric)
Presence of established B16.OVA tumors in the hosts

dependent variables

OTI T cell proliferation in tumor-draining lymph nodes

control variables

CD8+ T cells from OTI × B6.SJL-Ptprca mice
Positive selection of CD8+ T cells using anti-CD8+ MACS Beads
CFSE labeling of CD8+ T cells
Purity of CD8+ T cells (>87%) as assessed by positive staining with anti-CD8α, -Vα2, and -Vβ5 antibodies
Injection of approximately 2 × 10^6 CD8+ T cells i.v. into the hosts

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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