Protocol detail

Genetic Expression Analysis of Abdominal Fat

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Gene expression of abdominal fat samples in 825 individuals were analyzed with the Illumina Human HT-12 V3 (Illumina, Inc., San Diego, CA, USA) for the Muther study, as described previously.^{29 (link)} In all, 586 individuals were analyzed for expression association with the top metabolite using a random intercept linear regression including age, BMI, metabolite batch, expression batch and family relatedness.

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Pallister T., Jackson M.A., Martin T.C., Glastonbury C.A., Jennings A., Beaumont M., Mohney R.P., Small K.S., MacGregor A., Steves C.J., Cassidy A., Spector T.D., Menni C, & Valdes A.M. (2017). Untangling the relationship between diet and visceral fat mass through blood metabolomics and gut microbiome profiling. International Journal of Obesity (2005), 41(7), 1106-1113.

Publication 2017

Human HT-12 V3

Abdominal fat Gene expression Human

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Variable analysis

independent variables

Metabolite batch
Expression batch

dependent variables

Gene expression of abdominal fat samples

control variables

Family relatedness

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The independent variables manipulated by the researchers are the metabolite batch and expression batch. The dependent variable measured is the gene expression of abdominal fat samples. The control variables kept constant to ensure valid results are age, BMI, and family relatedness.

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