Tumor Experiments with Knockout Mice

For tumor experiments, wild-type (C57BL/6, Charles River Laboratories), CD30^KO OX40^KO (double knockout [dKO], C57BL/6), and CD30^KO OX40^KO Foxp3^KO (triple knockout [tKO], C57BL/6) female mice (6–8 wk old) were bred and maintained in the animal facilities at the Biomedical Services Unit (University of Birmingham, U.K.). The dKO and tKO mice were generated as described previously (22 (link), 23 (link)). In experiments where mAbs were injected, female C57BL/6 mice were used for experiments.

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Nawaf M.G., Ulvmar M.H., Withers D.R., McConnell F.M., Gaspal F.M., Webb G.J., Jones N.D., Yagita H., Allison J.P, & Lane P.J. (2017). Concurrent OX40 and CD30 Ligand Blockade Abrogates the CD4-Driven Autoimmunity Associated with CTLA4 and PD1 Blockade while Preserving Excellent Anti-CD8 Tumor Immunity. The Journal of Immunology Author Choice, 199(3), 974-981.

Publication 2017

C57BL/6

Animal C57bl mice Female Knockout mice Mabs Mice River Tumor

Corresponding Organization :

Other organizations : University of Birmingham, Uppsala University, Juntendo University, The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Genotype (wild-type, CD30^KO OX40^KO (dKO), CD30^KO OX40^KO Foxp3^KO (tKO))

dependent variables

Tumor experiments

control variables

Female mice (6-8 weeks old)
C57BL/6 mouse strain
Maintained in the same animal facilities

controls

Positive control: Female C57BL/6 mice for experiments where monoclonal antibodies (mAbs) were injected
Negative control: Not explicitly mentioned

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