Clinical Isolates of MSSA and MRSA

Bacterial strains used were clinical isolates of MSSA (n = 100) and MRSA (n = 100) isolated from St George’s Hospital, London. These isolates were collected from blood cultures, tissue fluid, or routine screening on skin of the patients in the South West London area. Most of these strains were isolated from bacteremia. Some were isolated as organ or skin colonization. Genotyping of these strains were performed previously (Hu et al., 2015 (link)). The isolates were grown in nutrient broth (Oxoid) or on trypticase soy agar (Oxoid) plates.

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Cunningham-Oakes E., Soren O., Moussa C., Rathor G., Liu Y., Coates A, & Hu Y. (2015). Nordihydroguaiaretic acid enhances the activities of aminoglycosides against methicillin- sensitive and resistant Staphylococcus aureus in vitro and in vivo. Frontiers in Microbiology, 6, 1195.

Publication 2015

nutrient broth trypticase soy agar

Agar Bacteremia Bacterial Blood cultures Mrsa Nutrient Patients Skin Strains Tissue fluid Trypticase soy

Corresponding Organization : St George's, University of London

Other organizations : University of Southampton

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Variable analysis

independent variables

Bacterial strains: MSSA (n = 100) and MRSA (n = 100)

dependent variables

Not explicitly mentioned

control variables

Bacterial isolates were grown in nutrient broth (Oxoid) or on trypticase soy agar (Oxoid) plates

controls

No positive or negative controls were explicitly mentioned in the provided information

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