Ultrastructural Changes in N. caninum-Infected Cells

HFF monolayers were grown in T25 culture flasks and were infected with 10⁷ N. caninum Spain-7 (NcSp-7) tachyzoites during 3 h at 37 °C and 5% CO₂. Subsequently, cultures were exposed to continuous treatment with 0.5 μM of each of DCQ, RMB054 or RMB060, and 1 µM RMB055 for 6 h, 9 h, and 48 h. Control cultures were treated with DMSO only. The specimens were fixed and processed for TEM as described earlier [14 (link),16 (link),17 (link)]. Samples were observed on a Philips CM 12 or a FEI Tecnai Spirit BioTwin TEM, both operating at 80 kV.

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Ramseier J., Imhof D., Hänggeli K.P., Anghel N., Boubaker G., Beteck R.M., Ortega-Mora L.M., Haynes R.K, & Hemphill A. (2023). In Vitro versus in Mice: Efficacy and Safety of Decoquinate and Quinoline-O-Carbamate Derivatives against Experimental Infection with Neospora caninum Tachyzoites. Pathogens, 12(3), 447.

Publication 2023

Tecnai Spirit BioTwin TEM

Dmso

Corresponding Organization : University of Bern

Other organizations : North-West University, Universidad Complutense de Madrid

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Variable analysis

independent variables

Treatment with 0.5 μM of each of DCQ, RMB054 or RMB060
Treatment with 1 μM RMB055

dependent variables

Ultrastructural changes in the infected HFF monolayers observed using TEM

control variables

HFF monolayers grown in T25 culture flasks
Infection with 10^7 N. caninum Spain-7 (NcSp-7) tachyzoites during 3 h at 37 °C and 5% CO2
Exposure to continuous treatment for 6 h, 9 h, and 48 h

controls

Positive control: DMSO-treated infected HFF monolayers
Negative control: Not mentioned

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