The pT2HB-PUHrT62 and pT2-β-actin plasmids were gifts from Professor Zongbin Cui in the Institute of Hydrobiology, Chinese Academy of Sciences (unpublished); T2KXIG47 (link) and pTol2mini (pDB739)59 (link) vectors were kindly provided by Professor Koichi Kawakami and Professor Stephen C Ekker respectively.
The rtTA DNA fragment was amplified from the pT2HB-PUHrT62 plasmid. The amplified rtTA DNA fragment was cloned into a pIRES2-EGFP plasmid (Clontech) to generate the prtTA-IRES2-EGFP construct. TRE3G promoter60 (link) was synthesized by the Biosune Company.
The core element of the chicken β-globin insulator was synthesized by the Biosune Company. Construction of ubi-pIDM vector: The ubi-promoter-rtTA-T2A-RFP-SV40 poly-A cassette was used to replace the β-actin promoter-rtTA-IRES-EGFP-SV40 poly-A cassette to form ubi-pIDM vector. The ubi promoter was from the Tg(ubi:loxP-DsRed-STOP-loxP-EGFP) plasmid clone as previously reported61 (link), and the T2A peptide was described previously37 .
The sequences of all primers are provided in Supplementary Table1 . The details of the construction are provided in Detailed protocol of Supplementary Materials.
The rtTA DNA fragment was amplified from the pT2HB-PUHrT62 plasmid. The amplified rtTA DNA fragment was cloned into a pIRES2-EGFP plasmid (Clontech) to generate the prtTA-IRES2-EGFP construct. TRE3G promoter60 (link) was synthesized by the Biosune Company.
The core element of the chicken β-globin insulator was synthesized by the Biosune Company. Construction of ubi-pIDM vector: The ubi-promoter-rtTA-T2A-RFP-SV40 poly-A cassette was used to replace the β-actin promoter-rtTA-IRES-EGFP-SV40 poly-A cassette to form ubi-pIDM vector. The ubi promoter was from the Tg(ubi:loxP-DsRed-STOP-loxP-EGFP) plasmid clone as previously reported61 (link), and the T2A peptide was described previously37 .
The sequences of all primers are provided in Supplementary Table
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