Gambierdiscus cheloniae CAWD232 collected from Rarotonga, The Cook Islands, in 2014, was cultured at 25 °C (±2 °C), 40–70 µmol m−2 s−1 photon irradiance (12:12 h light:dark cycle) [46 (link)]. The isolate was grown in f2/seawater (1:3; UV treated and filtered down to 0.22 µm using a Millipore filtration system; Millipore, Toronto, Canada) [47 (link)]. Consecutive 5 L monoclonal cultures (total of 100 L), equating to 1.6 × 108 cells, were harvested during the stationary phase of the growth cycle by centrifugation (3200× g, 10 °C, 10 min; Eppendorf 5810 R, Hamburg, Germany).
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