Flavonoid and Anthocyanin Analysis in Narcissus and Arabidopsis

Tepals, cornas from S1 to S4 stages of Narcissus tazetta and transformed callus tissue, as well transformed Arabidopsis plants were collected for analysis the contents of flavonoids and (pro)anthocyanin. HPLC were performed to analysis the extracts of flavonoids metabolism according to the description by our previous publication (Ren et al. 2017 (link); Yang et al. 2021 (link)). The flavonoids and anthocyanin standards including naringenin, dihydroquercetin, kaempferol, quercetin, lutein, Leucocyanidin, Catechin, and Cyanidin were purchased from Sigma-Aldrich (St. Louis, MO, USA), rutin and zeaxanthin were purchased from TCI (TCI Co., Ltd, Japan) and Sangon (Shanghai, China), respectively. All flavonoids and (pro)anthocyanin standards were prepared in MeOH/DMSO (1:1, v/v) and MTBE (containing 0.01% BHT), respectively, and stored at -20 °C before use.