Plasmid Cloning for Photoreceptor Labeling

pCIG2 and pCIG2 Lamin A were previously described [12 (link), 49 (link)]. A pCIG2 Lbr plasmid was generated by PCR amplifying Lbr from pMSCV-Flag-Lbr, generously provided by Peter Gaines [50 (link)] in order to remove the Flag tag. Primers were Lbr XhoI F: 5′-CACACTCGAGATGCCAAGTAGGAAGTTTGTTG-3′ and Lbr EcoRI R: 5′-CACAGAATTCTCAGTAAATGTAGGGGAATATG-3′. To mark rod photoreceptors, we utilized pRho-DsRed generously provided by Connie Cepko (Addgene #11156) [30 (link)]. Stable cell lines were generated using pBABE-puro-GFP-wt-lamin A (Addgene #17662) and pBABE-puro-GFP-Progerin (Addgene #17663) plasmids, generously shared by the Tom Misteli lab [51 (link)].