Additional metagenomes were generated from novel samples obtained from the following environmental sites: deep-sea sediment from Juan de Fuca Ridge in the Pacific Ocean off the coast of Canada; fumaroles and hot springs in the Azores; the Tatta Pani Hot Spring and Khewra Salt Mine, Pakistan; and four hot springs in Guangdong, China. More information on the sampling sites, including geographic coordinates and environmental parameters, is provided in the Supplementary Text .
Metagenomes were generated as previously described [59 (link)]. Briefly, genomic DNA was isolated from the samples using commercial kits with minor modifications. Quality and quantity of extracted DNA were determined with Nanodrop and Qubit spectrophotometers (Thermo Fisher Scientific Inc., Waltham, MA, USA). Metagenomic shotgun libraries of sheared DNA were prepared using the NEBNext® UltraTM DNA Library Prep Kit (New England BioLabs, Frankfurt am Main, Germany). Libraries were sequenced on an Illumina NexSeq550 instrument (Illumina, San Diego, CA, USA).
Metagenomes were generated as previously described [59 (link)]. Briefly, genomic DNA was isolated from the samples using commercial kits with minor modifications. Quality and quantity of extracted DNA were determined with Nanodrop and Qubit spectrophotometers (Thermo Fisher Scientific Inc., Waltham, MA, USA). Metagenomic shotgun libraries of sheared DNA were prepared using the NEBNext® UltraTM DNA Library Prep Kit (New England BioLabs, Frankfurt am Main, Germany). Libraries were sequenced on an Illumina NexSeq550 instrument (Illumina, San Diego, CA, USA).
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