Independent groups of rats were orally administered juanislamin or carbenoxolone (the reference compound) at different doses in a volume of 0.5 mL/100 g. Juanislamin was suspended in 0.05% Tween 80 and applied at 1–30 mg/kg. Carbenoxolone was dissolved in distilled water and delivered at 1–100 mg/kg. At 30 min post-treatment, 1 mL of ethanol was orally applied to all animals, regardless of weight. The animals were sacrificed 2 h later and the stomachs were immediately dissected, filled with 2% formaldehyde, and then placed in a container with the same solution for 5 min. Subsequently, each stomach was opened along the greater curvature and the area of the lesions was blindly measured by using a stereoscopic microscope (×10) equipped with an ocular micrometer. The sum of the area of the lesions of each stomach represents the ulcer index. Gastroprotection (%) was calculated according to:
where UIC and UIT are the ulcer indexes of the control and experimental groups, respectively [21 (link)].
where UIC and UIT are the ulcer indexes of the control and experimental groups, respectively [21 (link)].
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