Isolation of Fungi from Fish and Fish Feed

The isolation was done according to Alniaeem et al. (2015[2 ]) with slight modifications as follows: briefly, Dicloran rosebengal chloramphenicol media (DRBC) were used as recommended by Samson et al. (2004[39 ]). DRBC contained 5 g/L peptone, 10 g/L glucose, 1 g/L KH₂PO₄, 0.5 g/L MgSO₄ x 7H₂O, 0.1 % Dicloran (0.2 % in ethanol), 0.025 g/L rosebengal, 0.1 g/L chloramphenicol, and 15 g/L agar. Subsequently, 10 g of each sample from fish (abdominal muscle) and fish feed were crushed aseptically by a mortar and mixed well with 10 mL pepton water. Of this mixture, 0.1 mL was plated on DRBC media. Plates were incubated at 25 °C for 3-7 days.