BEAS-2B (human bronchial epithelial cell line) (ATCC® CRL9609™) was cultured with Bronchial Epithelial Cell Growth Basal Medium (BEBM) in tissue culture flasks coated with 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I, and 0.01 mg/mL bovine serum albumin (BSA). The cells were incubated at 37°C in a humidified environment with 5% CO2 [50 (link)].
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