Isolation and Identification of Phytochemicals from Nauclea pobeguinii

Compounds previously isolated from the bark of Nauclea pobeguinii included 3-acetoxy-11-oxo-urs-12-ene (1), p-coumaric acid (2), citric acid trimethyl ester (3), resveratrol (4), resveratrol β-_D-glucopyranoside (5) and strictosamide (6) (Fig. 1). Their isolation and identification were previously reported [25 (link)]. Tetracycline (TET), cefepime (CEP), ciprofloxacin (CIP), chloramphenicol (CHL), ampicillin (AMP), streptomycin (STR), kanamycin (KAN) (Sigma-Aldrich, St Quentin Fallavier, France) were used as reference antibiotics (RA). p-Iodonitrotetrazolium chloride (INT; Sigma-Aldrich) and Phenylalanine-Arginine-ß-Naphthylamide (PAßN; Sigma-Aldrich) were used as microbial growth indicator and efflux pumps inhibitor (EPI) respectively [27 (link), 28 (link)].Fig. 1

Chemical structures of the compounds isolated from Nauclea pobeguinii.1: 3-acetoxy-11-oxo-urs-12-ene; 2:p-coumaric acid; 3: citric acid trimethyl ester; 4: resveratrol; 5: resveratrol β-_D-glucopyranoside; 6: strictosamide