Regulation of CH25H by STAT1 in Alzheimer's Disease

Example 2

Our microarray data identified CH25H as a STAT1-regulated gene in AD conditions (FIGS. 3A and 3B). Furthermore, we confirmed the regulatory effect of STAT1 on CH25H by real-time PCR, Western Blot and ChIP assay (FIGS. 4A,4B,5A,5B). As shown in FIG. 4A which depicts real-time PCR results, CH25H mRNA expression is significantly reduced in STAT1 deficient mice. As shown in FIG. 4B which depicts Western Blot results, CH25H protein level is also significantly reduced in STAT1 deficient mice. Results from the ChIP assay in FIGS. 5A and 5B also showed that in STAT1 deficient mice, less STAT1 was binding to the CH25H gene promoter sequence. The reduced level of 25-OHC in STAT1 deficient mice further supported the notion that STAT1-CH25H axis controls brain 25-OHC level (FIG. 6A-6B).