Immunoblot Analysis of Signaling Proteins

Immunoblot analysis was performed as previously described 24 (link), 25 (link). The following antibodies were used: anti- FGFR2, from Santa Cruz Biotechnology, anti-GAPDH from Sigma-Aldrich, and anti-AKT, anti-phosphor-AKT, anti-ERK, anti-phosphor-ERK, anti-cyclinD1, anti-cyclinB1, and anti-phosphor-FGFR from Cell Signaling Technology. Secondary antibodies were from Sigma-Aldrich. Densitometry (Bio-Rad Laboratories) was applied to analyze the intensities of the respective protein bands.

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Li X., Nie C., Tian B., Tan X., Han W., Wang J., Jin Y., Li Y., Guan X., Hong A, & Chen X. (2019). miR-671-5p Blocks The Progression Of Human Esophageal Squamous Cell Carcinoma By Suppressing FGFR2. International Journal of Biological Sciences, 15(9), 1892-1904.

Publication 2019

anti- FGFR2 anti-GAPDH anti-AKT anti-phosphor-AKT anti-ERK anti-phosphor-ERK anti-cyclinD1 anti-cyclinB1 Densitometry

Antibodies Densitometry Fgfr Fgfr2 Gapdh Immunoblot analysis Phosphor Protein

Corresponding Organization :

Other organizations : Jinan University, Guangzhou Medical University, University of Hong Kong

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Variable analysis

independent variables

Antibodies used: anti-FGFR2, anti-GAPDH, anti-AKT, anti-phosphor-AKT, anti-ERK, anti-phosphor-ERK, anti-cyclinD1, anti-cyclinB1, and anti-phosphor-FGFR

dependent variables

Protein band intensities analyzed by densitometry

control variables

Immunoblot analysis performed as previously described in references 24 and 25

controls

No specific positive or negative controls mentioned

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