Immunoblot analysis was performed as previously described 24 (link), 25 (link). The following antibodies were used: anti- FGFR2, from Santa Cruz Biotechnology, anti-GAPDH from Sigma-Aldrich, and anti-AKT, anti-phosphor-AKT, anti-ERK, anti-phosphor-ERK, anti-cyclinD1, anti-cyclinB1, and anti-phosphor-FGFR from Cell Signaling Technology. Secondary antibodies were from Sigma-Aldrich. Densitometry (Bio-Rad Laboratories) was applied to analyze the intensities of the respective protein bands.
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