Generating Artificial Antigen-Presenting Cells

Both aAPC/mOKT3 and aAPC/A2 were derived from the human erythroleukemia cell line K562. The aAPC/mOKT3 expresses a membranous form of the anti-CD3 mAb (clone OKT3), as well as the costimulatory molecules CD80 and CD83^{25 (link)}. The aAPC/A2 expresses HLA-A2, CD80, and CD83^{27 (link)}. The human acute lymphoblastic leukemia cell line NALM6 was obtained from DSMZ (Braunschweig, Germany). The murine B-cell lymphoma cell line A20 and BALB/3T3 clone A31 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA). All cells were routinely checked for the presence of mycoplasma contamination using PCR-based technology.

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Kagoya Y., Nakatsugawa M., Saso K., Guo T., Anczurowski M., Wang C.H., Butler M.O., Arrowsmith C.H, & Hirano N. (2018). DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models. Nature Communications, 9, 1915.

Publication 2018

NALM6 BALB/3T3 clone A31

Aapc Acute lymphoblastic leukemia B cell lymphoma Balb 3t3 clone a31 Cell line Cells Clone Erythroleukemia Hla a2 Human Membranous Murine Mycoplasma Okt3

Corresponding Organization :

Other organizations : Princess Margaret Cancer Centre, University Health Network, University of Toronto, Structural Genomics Consortium

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Variable analysis

independent variables

AAPC/mOKT3
AAPC/A2

dependent variables

Not explicitly mentioned

control variables

The human acute lymphoblastic leukemia cell line NALM6
The murine B-cell lymphoma cell line A20
The BALB/3T3 clone A31
Mycoplasma contamination testing using PCR-based technology

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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