Sections of salivary glands with a thickness of 5 μm were obtained from paraffin blocks and stained with hematoxylin and eosin (H&E) for histological examination. In brief, sections were de-waxed in xylene for 10 min and rehydrated by sequential immersion in decreasing ethanol concentrations. Then, the sections were stained with H&E [109 (link)] and analyzed using a Leica DM5000 upright microscope (Leica Microsystems, Heidelberg, Germany). All sections were examined by two independent observers (F.C. and F.R.) in a blind manner, using coded slides and not knowing their source.
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