Genetic Manipulation of Regulatory T Cells

Foxp3^EGFPCre, Foxp3^YFPCre, CD4^Cre,
Rptor^fl/fl, Foxo1^fl/fl, R26^YFP and
Rag₁^–/– mice were obtained from the Jackson Laboratory.
Rictor^fl/fl mice were obtained from the Mutant Mouse Regional Resource Center.
R26^Foxo1AAA and Pfkfb3^fl/fl mice were generated as described
^{33 (link), 41 (link)}.
Foxp3^K276X, Foxp3^EGFP, CD45.1
Foxp3^EGFP, Foxp3^ΔEGFPiCre and their respective
crosses were backcrossed 8–10 generations on C57/BL6/J, excepted
Foxp3^ΔEGFPiCreFoxo1^Δ/Δ and
Foxp3^ΔEGFPiCreRictor^Δ/ΔFoxo1^Δ/Δstrain. A list of all mouse strains used is reported in Supplementary Table
1. Excepted when it was specified, 25–28 days old mice were used in this study. The mice were housed under
specific pathogen-free conditions and used according to the guidelines of the institutional Animal Research Committees at the
Boston Children’s Hospital.

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Charbonnier L.M., Cui Y., Victor E., Harb H., Lopez D., Bleesing J.J., Garcia-Lloret M.I., Chen K., Ozen A., Carmeliet P., Li M.O., Pellegrini M, & Chatila T.A. (2019). Functional Reprogramming of Regulatory T cells in the absence of Foxp3. Nature immunology, 20(9), 1208-1219.

Publication 2019

Foxp3EGFPCre Foxp3YFPCre CD4Cre Rptorfl/fl Foxo1fl/fl R26YFP andRag1–/–

Mice Pathogen Strains

Corresponding Organization :

Other organizations : Boston Children's Hospital, University of California, Los Angeles, Cincinnati Children's Hospital Medical Center, University of Utah, Marmara University, VIB-KU Leuven Center for Cancer Biology, Memorial Sloan Kettering Cancer Center, Harvard University

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Variable analysis

independent variables

Foxp3^EGFPCre
Foxp3^YFPCre
CD4^Cre
Rptor^fl/fl
Foxo1^fl/fl
Rictor^fl/fl
R26^Foxo1AAA
Pfkfb3^fl/fl
Foxp3^K276X
Foxp3^EGFP
Foxp3^ΔEGFPiCre
Foxo1^Δ/Δ
Rictor^Δ/Δ
Foxo1^Δ/Δ

dependent variables

Not explicitly mentioned

control variables

R26^YFP
Rag₁^–/–
CD45.1 Foxp3^EGFP

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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