Immunostaining of Brain Samples

Immunostaining of brain sections or dissociated cells was performed as described previously [47 (link)–49 (link)]. The following primary antibodies were used: rat anti-GFAP (#13–0300, Thermo Fisher Scientific, Waltham, MA, USA), rabbit anti-Iba1 (019–19,741, FUJIFILM Wako Pure Ceemical Corporation, Tokyo, Japen) antibody. Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Thermo Fisher Scientific, Waltham, MA, USA) were used to detect primary antibodies.

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Kim H.R., Lee Y.J., Kim T.W., Lim R.N., Hwang D.Y., Moffat J.J., Kim S., Seo J.W, & Ka M. (2020). Asparagus cochinchinensis extract ameliorates menopausal depression in ovariectomized rats under chronic unpredictable mild stress. BMC Complementary Medicine and Therapies, 20, 325.

Publication 2020

rat anti-GFAP Alexa Fluor dyes

Antibodies Antibody Brain Cells Dyes Gfap Rabbit

Corresponding Organization :

Other organizations : Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, Pusan National University, University of California, San Francisco

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Variable analysis

independent variables

Immunostaining of brain sections or dissociated cells

dependent variables

Expression of GFAP and Iba1 proteins

control variables

Immunostaining protocol described previously [47–49]
Appropriate secondary antibodies conjugated with Alexa Fluor dyes

positive controls

Not specified

negative controls

Not specified

Annotations

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